WebMar 1, 2004 · The immortalized cell lines, HMEC-1 (Ades et al., 1992) and BMEC-1 (Candal et al., 1996) were obtained from the Center for Disease Control (CDC, Atlanta, USA) and cultured in the same medium as HUVEC without ECGS. ECV304 cells were obtained from the American Tissue Culture Center (ATCC, Bethesda, MD) and cultured in M199 with … WebMay 3, 2024 · The isolated BMECs were cultured in a dish pre-coated with rat-tail collagen type 1 with endothelial cells medium supplement with growth factors. The cells were verified by confocal microscopy for morphology and tube formation by matrigel assay. We validate the cells' purity by flow cytometry, RT-qPCR, immunofluorescence staining, and ...
Human Cll Cell Line Mec 1 ATCC Bioz
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Human Cll Cell Line Mec 1 ATCC Bioz
WebFeb 15, 2007 · Aside from the loss of occludin and ZO-1 following BMEC exposure to CCL2, expression of caveolin-1 was also significantly down-regulated. 11 This additional chemokine-associated loss may further contribute to BBB alteration, because caveolin-1 is the major structural protein of caveolae, membrane microdomains critically involved in … WebFigure Legend Snippet: DZ2002 reduced ICAM-1 and VCAM-1 in vivo and in vitro. a Western blot of ICAM-1 and VCAM-1 in BLM-induced SSc mice skin (DZ2002 = 50 mg/kg). b Survival rate of HMEC-1 at different concentrations of DZ2002 treatment. c Evaluation of mRNA levels of molecules associated with the upregulation of endothelial adhesion … steven priddle anchorage