WebDec 19, 2011 · 分析测试百科 [分享帖]cck8试验~。~菜鸟篇(转载)我没有做过mtt实验,但其实原理及目的都是一样的(反应机理不一样)。都说cck8简单点而且数据更稳定,所以就用了这个试剂盒。cck8的说明书大家一定要认真阅读,里面很多细节的问题都有提到。而且说明书里提供的一些关于各种细胞的种板数都很 ... WebJun 24, 2024 · The IC50 of nab-paclitaxel was 3.68 μmol/L in hGCO1, 2.41 μmol/L in hGCO2 and 2.91 μmol/L in hGCO3, which was significantly lower than those of 5-FU …
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Webreduction in the presence of an electron carrier, as shown in Figure 1. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit - 8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WebFit the ratio of two IC50 values directly From the results in step 8, you can compute what you want to know -- the ratio of the two EC50 values. But Prism can calculate this value directly. Go back to the analysis parameters dialog, and on the Fit tab, change the equation to "EC50 shift" from the "Dose-response - Special" group of equations. blackburn diocese safeguarding training
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WebJul 9, 2024 · Moreover, the CCK8 results showed that the increased IC50 values in CDYL-overexpressing cells were rescued by GSK126 (Figure (Figure6B). 6 B). Similarly, xenograft experiments also showed that the increased xenograft growth and tumour volumes observed after CDYL overexpression in H69 cells were significantly inhibited by the combination of ... Web取对数生长期的A549细胞,胰酶消化后,按照每孔70%左右的比例铺于6孔板中,培养过夜后,按照Lipo8000说明书要求转染pcDNA3.1-GPX4及pcDNA3.1质粒至A549细胞,根据转染质粒不同分为阴性对照组(pcDNA3.1组)以及质粒过表达组(pcDNA3.1-GPX4组)进行后续的CCK8及蛋白质印迹技术 ... WebSep 10, 2024 · cck8试剂盒 ; 化合物库; 新闻 ... 其计算方法为添加烷基化剂甲基甲烷磺酸盐(MMS)后控制生长的IC50与添加PARP抑制剂后MMS的IC50之比。使用HeLa B细胞,奥拉帕尼在固定的200 nM浓度下进行MMS筛选。用于检测Olaparib对SW620结直肠细胞系的作用,使用的浓度分别为1、3、10 ... gallant bob horse