2024 Jeg-3 cell

Jeg-3 cell

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August undefined, 2024

Web1 dic 2024 · In contrast, the JEG-3 cell line fails to undergo substantial fusion. Therefore, BeWo and JEG-3 cells were used to identify a set of genes responsible for trophoblast fusion. Cells were treated with forskolin for 48 h to induce fusion. RNA was extracted, hybridised to Affymetrix HuGene ST1.0 arrays and analysed using system biology. Web19 lug 2024 · For Jeg-3 cells the reduced glucose transfer rates cannot be explained by differences in proliferation, metabolic activity or cell-to-cell junctions since they displayed similar proliferation rates as BeWo b30 and JAR cells, similar metabolic activity as JAR cells and comparable and high TEER values as ACH-3P.

Human choriocarcinoma cell line JEG-3 produces and …

Web12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC … Webhuman H1299 cells-Function assay: 7 days: Inhibition of human USP1/UAF1 in human H1299 cells assessed as reduction in colony formation after 7 days, EC50=3 μM: 25229643: human JEG3 cells-Function assay-Inhibition of aromatase in human JEG3 cells by scintillation spectrometry, IC50=0.0015 μM: 20148564: human K562 cells: 1 mM: … protected mail

A comparative study of five physiological key parameters ... - Nature

WebCell line: JEG-3 : DSMZ no.: ACC 463: Species validation: human (Homo sapiens)Cell type: choriocarcinoma : Origin: established from a human gestational choriocarcinoma which … WebMaintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve … WebCaco-2 and Jeg-3 cells were left NI or Lm-infected for 17 h (Inf). Flow cytometry analysis of unfixed cells showed that viability was not affected by infection (Figure S1A). In Caco-2 cells we detected 75% of infected and 25% of uninfected bystander cells (Figure S1B), while 57% of Jeg-3 cells were infected (data not shown). Flow protected maintenance ltd

HLA-G gene editing in tumor cell lines as a novel alternative

JEG-3 cell culture supernatants cause reduced interferon-gamma …

Web13 mar 2024 · The culture of JEG-3 cells (a human choriocarcinoma cell line) has been described previously . To study the cytotoxic effect of α-solanine on JEG-3 cells, cells were cultured in 96-well plates overnight, followed by treatment with different concentrations of α-solanine (10, 20, 30, 40 and 50 μM) or vehicle (0.5% v/v DMSO) for 24 WebMethod of study: A human choriocarcinoma cell line JEG-3 was used to test the effects of its culture supernatants on the proliferation and cytokine production in the MLRs. The … reshade wireframe shaderWebHuman choriocarcinoma trophoblastic cells (JEG-3) were used as positive control for the constitutive expression of HLA-G [53]. The cells were cultured in RPMI medium (Sigma-Aldrich, St. Louis, M, USA) with glutamine and antibiotics with 10 % FBS and maintained at 37 Cina sterile humid atmosphere under 5 % CO 2 and 95 % O 2.No reshade winter

"WebJEG-3 cells have been demonstrated to be a suitable cell line for modeling the effects of insulin on trophoblast metabolism and placental function.30 Hence, to mimic the effects … " - Jeg-3 cell

Jeg-3 cell

WebNorfluoxetine increased MMP-9 activity in JEG-3 cells by 2.0% at 0.03 μM and by 43.9% at 3 μM, but decreased MMP-9 activity in HIPEC cells by 63.7% at 3 μM. Sertraline at 0.03 μM increased mRNA level of TIMP-1 in JEG-3 cells by 36% and that of ADAM-10 by 85% and 115% at 0.3 and 3 μM, respectively. Web1 mag 2002 · JEG-3 cells (5×10 5 cells in 30 mm dishes) incubated overnight with retinol (with or without ethanol or 4MP) and the conditioned media were placed on the F9-1.8 …

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Web29 nov 2024 · BeWo and JEG-3 cells were seeded in 35-mm dishes at a density of 500 × 10 3 cells/well in the presence of a complete medium for 6–8 h prior to serum starvation … WebJEG-3 is a hypertriploid, clonally-derived, human cell line with epithelial morphology that was isolated from the Woods strain of the Erwin-Turner tumor. This cell line is … The ATCC Contact Us web page helps ATCC website visitors get help with their … To ensure your research is high quality, use authenticated ATCC cells. ATCC has … ATCC addresses this need by providing rigorous quality control in its … See an overview of the ATCC stem cell collection and describe how these … ATCC is working to harmonize our Safety Data Sheet (SDS) distribution with …

WebWhen JEG-3 cells were stimulated with rhIFN-γ prior to co-culture, CD4 + HLA-G + T cells were significantly increased, and IFN-γ and TNF-α elevated. Taken together, the results indicate that JEG-3 cells upregulate regulatory T cell phenotypes and modulate the level of pro-inflammatory cytokines, which might be important mechanisms in the tumor … Web12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC on JEG-3 edited cells.

Web19 lug 2024 · Comparing overall hCG levels among the four cell models, Jeg-3 cells produced the highest total amount of hCG, while JAR cells displayed the least sensitivity … Web2.3.2 Trophoblast cell lines. Choriocarcinoma-based trophoblast cell lines have been extensively utilized to mimic the placental barrier for screening NP toxicity, translocation and uptake (see Table 2) [42, 95, 101]. They include BeWo, JAR and JEG-3, of which BeWo cell line is the most commonly used model [101].

WebMethods: HTR-8/SVneo, BeWo, JEG-3 and JAR were analyzed for epithelial and mesenchymal markers using immunofluorescence, real time PCR and Western blot. Results: Our results showed that HTR-8/SVneo cell line contains two populations of cells suggesting the presence of trophoblast and stromal/mesenchymal cells. While all cells …

Web1 dic 2024 · In contrast, the JEG-3 cell line fails to undergo substantial fusion. Therefore, BeWo and JEG-3 cells were used to identify a set of genes responsible for trophoblast … reshade with addonsWeb30 ott 2024 · Transporters are membrane proteins that regulate the absorption, distribution, and excretion of substrates in all cells of the body. ... The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells. Toxicol. Lett. 2013, 218, 200–206. [Google Scholar] Chen, Z.S ... reshade wireframeWebseed out at ca. 1 x 10 6 cells/80 cm 2; maintain at ca. 10 x 10 6 cells/175 cm 2; split confluent culture up to 1:10 every 3-4 days using trypsin/EDTA. Incubation: at 37 °C with 5% CO 2. Doubling time: about 24 hours. Harvest: cell harvest of ca. 25 x 10 6 cells/175 cm 2. Storage: frozen with 70% medium, 20% FBS, 10% DMSO. reshade with addon supportWebResults: In this study, we found that: (1) the invasive ability of JEG-3 cells was inhibited sufficiently (P < 0.05) after JEG-3 cells were transfected by Fe3O4-dextran-anti-βHCG carrying Hpa ASODN; (2) after JEG-3 cells were transfected by Fe3O4-dextran-anti-βHCG carrying Hpa ASODN at 48 and 72 hours, ... reshadewinter.iniWeb31 mar 2024 · JEG-3 cells prepared in our incubation conditions were renamed JEG-Tox cells. As placental alterations are mainly triggered by uncontrolled apoptosis, we second … reshade without network detectionWeb1 gen 2024 · JEG-3 was further evolved to AC1-1-cells, as a hypoxanthine-guanine phosphoribosyltransferase-negative mutant [10]. AC1-1 cells were transformed into two … reshade witcher 3WebWhen JEG-3 cells were stimulated with rhIFN-γ prior to co-culture, CD4 + HLA-G + T cells were significantly increased, and IFN-γ and TNF-α elevated. Taken together, the results … reshade with dxvk