Peasy-t5 zero cloning kit
WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebTransGen Biotech pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector …
Peasy-t5 zero cloning kit
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WebJul 30, 2024 · Recombination plasmid of HA gene of H5 subtype has been constructed using the pEASY™-T5 Zero Cloning Kit. The plasmid was cleaved using a suitable restriction enzyme. The digested plasmid was purified with a DNA recovery kit, the concentration was measured with a Nano Drop nucleic acid quantifier, and then an appropriate amount was … WebNov 9, 2024 · Cloning of CsA-IPT gene by 3′ and 5′ rapid amplification of ... China), respectively. Purified PCR products were cloned into the pMD18-T vector (pEASY-T5 Zero Cloning Kit, TransGen Biotech, Beijing, China), then transformed into the Trans1-T1 phage resistant chemically competent cell (TransGen Biotech). DNA sequencing was performed …
WebOct 4, 2024 · Each PCR product was inserted into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing), and more than five clones per reaction were sequenced (Ykang, Hangzhou, China). The 5’ and 3’RACE fragments amplified using the corresponding primers were then assembled using DNAMAN 8.0 software (Lynnon Biosoft, Canada). WebpEASY-T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. All the sequences of the primers used in this study are shown in
Web产品详情介绍. 该载体通过自杀基因表达与否筛选阳性重组子。. 当载体与片段连接成功时，自杀基因无法正确表达，包含重组子的细胞可以正常生长；当载体与片段连接不成功时，自杀基因正确表达，包含载体的细胞无法生长，即“Zero”背景。. 适用于TA克隆 ... WebProduct Details. pEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection.
WebpEASY® -T5 Zero Cloning Kit pEASY®-T5 Zero Cloning Vector contains a suicide gene. Pricing & AvailabilityDownload CatalogueAdd to wishlist View More Details Add to Cart pEASY® -Uni Seamless Cloning and Assembly Kit No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced.
WebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … olivia rodrigo unfollows taylor swiftWebMay 28, 2024 · The products were ligated to T5 zero cloning vector and transformed with Trans1-T1 into Competent cells by the heat-shock process at 42°C for 30 s (pEASY®-T5 Zero Cloning Kit). Three colonies were randomly picked from each transformation and incubated in Luria-Bertani liquid media at 37°C overnight under continuous shaking. One … olivia rodrigo unreleased songs chordsWebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step. The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants ... is amazon alexa free on fitbitWebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Features; Plasmids; ... pEASY-T5 Zero (linearized) pKF 18k-2. pSMART LCKan (linearized) CMV promoter. pEX-1. pKF 19k-2. pSP64 Poly(A) GAL1 promoter. pEX-A. pKOV ... olivia rodrigo traitor lyrics videoWebIntroduction Product Overview Description of the system The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts … olivia rodrigo\u0027s motherWebpEASY® -Blunt Zero Cloning Kit 目录号： CB501-01 单 价： ￥780 规格： 20 rxns 60 rxns 数量： - + 说明书 载体信息 质检报告 立即下单 加入购物车 产品详情介绍 该载体通过自杀基 … is amazon alexa downWebpEASY® -T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed … olivia rodrigo t shirts target