網頁The concept of using stearylated peptides may aid in the development of more efficient nonviral gene vectors. AB - The internalization mechanisms associated with octaarginine … 網頁2024年4月30日 · In the last few years, a new stearylated peptide, STR-KV (STR-HHHKKKVVVVVV) has been developed and shown to cross the plasma membrane through a direct translocation pathway, achieving an 80% to 87% gene knockdown in three different cell lines [ 109 ].

The nanoparticulation by octaarginine-modified liposome …

網頁由於此網站的設置，我們無法提供該頁面的具體描述。 網頁2024年3月6日 · Stearylated octaarginine (STR-R8) was synthesized by Scrum Inc. (Tokyo, Japan). The mouse colon cancer cell line, colon26, was provided by the RIKEN BRC under the National Bio-Resource Project of the MEXT/AMED, Japan. Colon26 cells stably expressing the luciferase gene (colon26-Luc) were established in our laboratory. peripheral nerve sensation arm

Cell-Penetrating Peptides Delivering siRNAs: An Overview

網頁2014年8月4日 · The packaging of siRNA was performed according to an adapted protocol from DNA transfection experiments. 26 An amount of 1.5 μL of Stearyl-R8 was diluted in 50 μL of Neurobasal medium without supplements and combined with 10 pmol of siRNA in 50 μL of Neurobasal medium without supplements after 5 minutes' incubation at room … 網頁Preparation and Characterization of Octaarginine-modified Liposomes—A series of R8-Lip containing various concentrations of STR-R8 peptide was prepared. In this preparation, … 網頁2006年2月10日 · Stearylated-octaarginine was synthesized as described previously ( 25 ). Other chemicals were purchased from Wako Chemicals (Osaka, Japan). Plasmid DNA Pcmv-luc-encoding luciferase was prepared by using an EndFree Plasmid Mega Kit (Qiagen, Hilden, Germany). NIH3T3 cells were obtained from the American Type Culture … peripheral nerve sensation