WebApoptosis was triggered in Jurkat cells with staurosporine and then flow cytometry was used to measure three aspects of mitochondrial damage: (1) cytochrome c release (with the digitonin assay and a commercially available kit based on the same principle), using a DNA-binding dye to define cell cycle stage; (2) loss of mitochondrial cardiolipin ... WebThis can be detected using flow cytometry using three fluorescent dyes: JC-1 (Figure 3A), TMRE (Figure 3B), and TMRM . These dyes are able to enter healthy mitochondria where they aggregate and brightly fluoresce. As apoptosis occurs, the mitochondrial membrane potential collapses and the dyes disperse throughout the cell.
A comparison of three flow cytometry methods for evaluating ... - PubMed
WebMitoProbe™ TMRM Kit for Flow Cytometry Catalog No. M20036 Product information Presence of a mitochondrial membrane potential (ΔΨm), the electrochemical gradient between the interior and exterior of the mitochondria, is a sign of healthy cells. As a cationic and lipophilic fluorescent dye, TMRM can be used to detect WebTetramethylrhodamine ethyl ester perchlorate (TMRE) is used to study the effects of high glucose in inducing mitochondrial morphology and metabolic changes. [ 2] TMRE … scotch brite dishwand target
Flow Cytometry Gating for Beginners Proteintech Group
WebTMRM Assay For Use With (Equipment) Flow Cytometer Product Type Flow Cytometry Assay Kit Emission 561 Format Vial (s) Shipping Condition Wet Ice Contents & Storage 1 … WebAnalysis of vascular permeability using the modified Evans Blue Dye ... (TMRE) in HSCs of aged mice following PBS/NTN1 treatment. Bar graphs demonstrating no significant changes in % of HSCs with high MMP i, or ... Multi-lineage engraftment was monitored post-transplantation by flow cytometry analysis of red blood cell (RBC)-lysed peripheral ... Web(B) Flow cytometry analysis of PBMCs incubated at 37°C with 64 µg/mL NT B 700 at different time points (1, 4, 24 and 48 h) washed and then stained LTG, TMRE, DCF and MDC. Fold of change, calculated as the ratio between cells incubated with NPs and control cells (w/o NPs), from the flow cytometry histograms is shown as a function of time in ... prefer working from home